Combined Bone Mesenchymal Stem Cells and Olfactory Ensheathing Cells Transplantation Promote Neural Repair Associated with CNTF Expression in Traumatic Brain Injured Rats
Abstract:This study examined the role of bone mesenchymal stem cells (BMSCs) and olfactory ensheathing cells (OECs) co‐grafting on neural function and underlying molecular mechanisms in acute stage of traumatic brain injury (TBI) rats. Eighty Sprague Dawly (SD) female rats were randomly divided into five groups (n=16 per category): the sham operated group (Sham), weigh‐drop induced traumatic brain injury group (TBI), BMSCs transplantation group (BMSC), OECs transplantation group (OEC), and co‐transplantation group (CO). Eight rats were randomly selected from each group for behavioral and morphological assessment. Another category (n=8 rats) was employed in the genetic expression detection. BMSCs were isolated from GFP mouse and identified by CD44 antibody, while OECs were isolated from the SD rats, identified by P75 antibody and labeled by Hoechst33342. They were then transplanted into the surrounding tissue of epicenter of TBI rats. The result of neurological severity scores (NSS) revealed that BMSCs or OECs transplantation alone and BMSCs, OECs co‐grafting significantly ameliorated the neurological function of TBI rats. Quantitative immunohistochemical analysis showed that graft‐recipient animals possessed dramatically more neurons and regeneration axons and smaller amounts of astrocytes than controls 14 days post transplantation (p<0.05). However, the expressional level of ciliary neurotrophic factor (CNTF) significantly decreased in co‐grafting group by RT‐PCR (p<0.05) and the Janus kinase/signal transducer and activator of transcription (JaK/STAT) pathway was significantly activated at 7 days of cell transplantation (p<0.05). This study firstly reported the role of co‐transplantation of BMSCs and OECs in the therapy of TBI and explored its potential molecular mechanism, therefore providing the important morphologic and molecular biological evidence for the clinical application of BMSCs and/or OECs transplantation in TBI.
Appeared or available online: Fri Mar 07 00:00:00 UTC 2014