A Physiological Pattern of Oxygenation Using Perfluorocarbon-Based Culture Devices Maximizes Pancreatic Islet Viability and Enhances β-Cell Function
Authors: Fraker, Chris A.; Cechin, Sirlene; Álvarez-Cubela, Silvia; Echeverri, Felipe; Bernal, Andrés; Poo, Ramón; Ricordi, Camillo; Inverardi, Luca; Domínguez-Bendala, Juan
Source: Cell Transplantation, Volume 22, Number 9, 2013 , pp. 1723-1733(11)
Publisher: Cognizant Communication Corporation
Abstract:Conventional culture vessels are not designed for physiological oxygen (O2) delivery. Both hyperoxia and hypoxia—commonly observed when culturing cells in regular plasticware—have been linked to reduced cellular function and death. Pancreatic islets, used for the clinical treatment of diabetes, are especially sensitive to sub- and supraphysiological O2 concentrations. A result of current culture standards is that a high percentage of islet preparations are never transplanted because of cell death and loss of function in the 24‐48 h postisolation. Here, we describe a new culture system designed to provide quasiphysiological oxygenation to islets in culture. The use of dishes where islets rest atop a perfluorocarbon (PFC)-based membrane, coupled with a careful adjustment of environmental O2 concentration to target the islet physiological pO2 range, resulted in dramatic gains in viability and function. These observations underline the importance of approximating culture conditions as closely as possible to those of the native microenvironment, and fill a widely acknowledged gap in our ability to preserve islet functionality in vitro. As stem cell-derived insulin-producing cells are likely to suffer from the same limitations as those observed in real islets, our findings are especially timely in the context of current efforts to define renewable sources for transplantation.
Document Type: Research Article
Affiliations: Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, USA
Publication date: 2013-09-11
- Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.
- In this: publication
- By this: publisher
- In this Subject: Anatomy & Physiology , Biology , Biotechnology , Pharmacology , Surgery
- By this author: Fraker, Chris A. ; Cechin, Sirlene ; Álvarez-Cubela, Silvia ; Echeverri, Felipe ; Bernal, Andrés ; Poo, Ramón ; Ricordi, Camillo ; Inverardi, Luca ; Domínguez-Bendala, Juan