Seventy-Two-Hour Preservation, Resuscitation, and Transplantation of an Isolated Rat Heart With High Partial Pressure Carbon Monoxide Gas (PCO = 400 hPa) and High Partial Pressure Carbon Dioxide (PCO₂ = 100 hPa)

Authors: Hatayama, Naoyuki; Yoshida, Yu; Seki, Kunihiro

Source: Cell Transplantation, Volume 19, Numbers 6-7, 2010 , pp. 707-712(6)

Publisher: Cognizant Communication Corporation

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Abstract:

The cardiac cavity of an isolated rat heart was filled with a Krebs-Henseleit (KH) solution, and the heart was hung in a high-pressure chamber. After the high-pressure chamber had been filled with a mixed gas (PCO = 400 hPa, PCO₂ = 100 hPa, PO₂ = 900 hPa, PHe = 5600 hPa) and preserved for 72 h, we performed a cervical ectopic heart transplantation on a recipient rat and resuscitated the preserved heart. This is the first incidence in the world of a mammalian organ having been successfully preserved and resuscitated after 72 h via a desiccation method.

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Keywords: Isolated rat heart; Perfluorocarbon (PFC); Preservation; Desiccation; Resuscitation; Heterotrophic transplantation

Document Type: Research article

DOI: http://dx.doi.org/10.3727/096368910X508807

Affiliations: 1: , Tokyo,

Publication date: 2010-06-01

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Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.