Seventy-Two-Hour Preservation, Resuscitation, and Transplantation of an Isolated Rat Heart With High Partial Pressure Carbon Monoxide Gas (PCO = 400 hPa) and High Partial Pressure Carbon Dioxide (PCO2 = 100 hPa)
Abstract:The cardiac cavity of an isolated rat heart was filled with a Krebs-Henseleit (KH) solution, and the heart was hung in a high-pressure chamber. After the high-pressure chamber had been filled with a mixed gas (PCO = 400 hPa, PCO2 = 100 hPa, PO2 = 900 hPa, PHe = 5600 hPa) and preserved for 72 h, we performed a cervical ectopic heart transplantation on a recipient rat and resuscitated the preserved heart. This is the first incidence in the world of a mammalian organ having been successfully preserved and resuscitated after 72 h via a desiccation method.
Document Type: Research Article
Affiliations: Resonance Club Co., Ltd., Tokyo, Japan
Publication date: June 1, 2010
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