Open Access

Protocols for Hematopoietic Stem Cell Expansion From Umbilical Cord Blood

Authors: Koestenbauer, Sonja; Zisch, Andreas; Dohr, Gottfried; Zech, Nicolas H.

Source: Cell Transplantation, Volume 18, Numbers 10-11, October 2009 , pp. 1059-1068(10)

Publisher: Cognizant Communication Corporation

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Abstract:

The reconstitution of adult stem cells may be a promising source for the regeneration of damaged tissues and for the reconstitution of organ dysfunction. However, there are two major limitations to the use of such cells: they are rare, and only a few types exist that can easily be isolated without harming the patient. The best studied and most widely used stem cells are of the hematopoietic lineage. Pioneering work on hematopoietic stem cell (HSC) transplantation was done in the early 1970s by ED. Thomas and colleagues. Since then HSCs have been used in allogenic and autologous transplantation settings to reconstitute blood formation after high-dose chemotherapy for various blood disorders. The cells can be easily harvested from donors, but the cell number is limited, especially when the HSCs originate from umbilical cord blood (UCB). It would be desirable to set up an ex vivo strategy to expand HSCs in order to overcome the cell dose limit, whereby the expansion would favor cell proliferation over cell differentiation. This review provides an overview of the various existing HSC expansion strategies—focusing particularly on stem cells derived from UCB—of the parameters that might affect the outcome, and of the difficulties that may occur when trying to expand such cells.

Keywords: Cell therapy; Cord blood; Ex vivo expansion; Stem cells

Document Type: Review Article

DOI: http://dx.doi.org/10.3727/096368909X471288

Affiliations: Institute for Cell Biology, Histology and Embryology, Centre of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria. sonja.koestenbauer@meduni-graz.at

Publication date: October 1, 2009

More about this publication?
  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.
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