@article {Noguchi:May :0963-6897:563,
	author = "Noguchi, Hirofumi and Oishi, Koichi and Ueda, Michiko and Yukawa, Hiroshi and Hayashi, Shuji and Kobayashi, Naoya and Levy, Marlon F. and Matusmoto, Shinichi",
	title = "Establishment of Mouse Pancreatic Stem Cell Line",
	journal = "Cell Transplantation",
	volume = "18",
	number = "5-6",
	year = "May ",
	abstract = "β-Cell replacement therapy via islet transplantation is a promising possibility for the optimal treatment of type 1 diabetes. However, such an approach is severely limited by the shortage of donor organs. Pancreatic stem/progenitor cells could become a useful target for β-cell replacement therapy in diabetic patients because the cells are abundantly available in the pancreas of these patients and in donor organs. In this study, we established a mouse pancreatic stem cell line without genetic manipulation. The duct-rich population after islet isolation was inoculated into 96-well plates in limiting dilution. From over 200 clones, 15 clones were able to be cultured for over 3 months. The HN#13 cells, which had the highest expression of insulin mRNA after induction, expressed PDX-1 transcription factor, glucagon-like peptide-1 (GLP-1) receptor, and cytokeratin-19 (duct-like cells). These cells continue to divide actively beyond the population doubling level (PDL) of 300. Exendin-4 treatment and transduction of PDX-1 and NeuroD proteins by protein transduction technology in HN#13 cells induced insulin and pancreas-related gene expression. This cell line could be useful for analyzing pancreatic stem cell differentiation. Moreover, the isolation technique might be useful for identification and isolation of human pancreatic stem/progenitor cells.",
	pages = "563-571",
	url = "http://www.ingentaconnect.com/content/cog/ct/2009/00000018/F0020005/art00012",
	keyword = "Pancreatic stem cell, PDX-1, BETA2/NeuroD, Exendin-4, Pancreatic duct, Protein transduction domain"
}