Beneficial Effect of the Nonpeptidyl Low Molecular Weight Radical Scavenger IAC on Cultured Human Islet Function
Authors: Mancarella, Rita; Del Guerra, Silvia; Masini, Matilde; Bugliani, Marco; Valgimigli, Luca; Pedulli, Gian Franco; Paolini, Moreno; Canistro, Donatella; Armando, Andrea; Soleti, Antonio; Filipponi, Franco; Mosca, Franco; Boggi, Ugo; Del Prato, Stefano; Marchetti, Piero; Lupi, Roberto
Source: Cell Transplantation, Volume 17, Numbers 10-11, October 2008 , pp. 1271-1276(6)
Publisher: Cognizant Communication Corporation
Abstract:We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan donors by collagenase digestion were purified by density gradient centrifugation. After the isolation, islets were either exposed or not exposed for 7 days to 10 μmol/L IAC. We found that IAC markedly reduced oxidative stress and ameliorated islets function. These results suggest that the use of IAC could be an interesting pharmacological approach for the treatment of the islets before transplantation.
Document Type: Research Article
Publication date: October 1, 2008
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- In this Subject: Anatomy & Physiology , Biology , Biotechnology , Pharmacology , Surgery
- By this author: Mancarella, Rita ; Del Guerra, Silvia ; Masini, Matilde ; Bugliani, Marco ; Valgimigli, Luca ; Pedulli, Gian Franco ; Paolini, Moreno ; Canistro, Donatella ; Armando, Andrea ; Soleti, Antonio ; Filipponi, Franco ; Mosca, Franco ; Boggi, Ugo ; Del Prato, Stefano ; Marchetti, Piero ; Lupi, Roberto