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Glycocalyx Damage Estimated Using Colloidal Iron Staining

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Authors: Zhang, Daoxin 1 ; Isaka, Yoshitaka 2 ; Imamura, Ryoichi 3 ; Ichimaru, Naotsugu 3 ; Shi, Yi 3 ; Imai, Enyu 4 ; Tian, Ye 5 ; Ohtsuka, Aiji 6 ; Takahara, Shiro 7 ; 

Source: , 1 January 2008, vol. 17, no. 1-2, pp. 159-163(5)

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DOI: https://doi.org/10.3727/000000008783907134

Anionic constituents in the peritubular capillary basement membranes and the glomerular endothelial cells have been demonstrated to function as a size- and charge-selective barrier. Cationic colloidal iron staining of human biopsy specimens revealed a glycocalyx on the surface of the glomerular basement membrane (GBM), peritubular capillary (PTC) endothelial cells, and brush border of the tubular epithelial cells of normal kidney. However, the glycocalyx was abolished in the PTC wall of C4d-positive acute humoral rejected kidney, and in the GBM as well as the PTC wall of a chronic, allograft, nephropathy kidney. In addition, cyclosporine eliminated the glycocalyx in the PTC wall, while treatment with heparin inhibited the elimination of the PTC glycocalyx. In conclusion, the glycocalyx on the surface of the GBM and PTC is an important component in the endothelial cell barrier.

Keywords: Chronic allograft nephropathy; Cyclosporine; Glycocalyx; Humoral rejection; Transplantation

Document Type: Research Article

Affiliations: 1: Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Osaka, Japan, Beijing Friendship Hospital, The Capital Medical University, Beijing, China 2: Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Osaka, Japan, Department of Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan 3: Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan 4: Department of Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan 5: Beijing Friendship Hospital, The Capital Medical University, Beijing, China 6: Department of Human Morphology, Okayama University School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan 7: Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Osaka, Japan

Publication date: 01 January 2008

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