ET-Kyoto Solution Plus Dibutyryl Cyclic Adenosine Monophosphate Is Superior to University of Wisconsin Solution in Rat Liver Preservation
Abstract:ET-Kyoto solution (ET-K) is an extracellular-type organ preservation solution containing the cytoprotective disaccharide, trehalose. A previous study reported the supplement of dibutyryl cyclic adenosine monophosphate (db-cAMP) in conventional ET-K to attenuate lung ischemia-reperfusion injury. In this study, the efficacy of this modified ET-K for liver preservation was investigated by comparison with University of Wisconsin solution (UW). ET-K was supplemented with db-cAMP (2 mmol/L). Lewis rats were randomly assigned to two groups, and liver grafts were flushed and stored at 4°C for 24 h with ET-K or UW before syngeneic liver transplantation. The graft function and histological changes at 4 h posttransplant as well as 7-day survival were evaluated. Recipient rat survival rate was significantly higher in the ET-K group than in the UW group. Preservation in ET-K resulted in a significant reduction in serum parenchymal transaminase level and promotion of bile production in comparison with UW. The serum hyaluronic acid level, an indicator of sinusoidal endothelial cell injury, was significantly lower after ET-K preservation than that in UW. Histologically, at 4 h after transplantation, the liver grafts preserved in UW solution demonstrated a greater degree of injury than those in ET-K, which appeared to be apoptosis, rather than necrosis. The continuity of the sinusoidal lining was better preserved in ET-K than in UW. In conclusion, ET-K supplemented with db-cAMP is superior to UW in rat liver preservation. This modified ET-K might therefore be a novel candidate for the procurement and preservation of multiple organs.
Document Type: Research Article
Affiliations: 1: Department of Organ Preservation Technology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan 2: Horizontal Medical Research Organization, Transplant Tolerance Unit, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan 3: Department of Pathology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan 4: Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan 5: Institute of Biomedical Research and Innovation, Foundation of Biomedical Research and Innovation, Kobe 650-0047, Japan
Publication date: 2008-01-01
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