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Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function

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Abstract:

For islet transplantation, it is important to obtain an available islet mass adequate for diabetes reversal from a single donor pancreas. A recent report demonstrated that the use of M-Kyoto solution instead of UW solution improved islet yields in the two-layer method for pancreas preservation. The present study investigated whether the ductal injection of a large volume of preservation solution (UW and M-Kyoto solution) before pancreas storage improves islet yields. Islet yield both before and after purification was significantly higher in the ductal injection (+) group compared with the ductal injection (−) group. TUNEL-positive cells in the ductal injection (+) group were significantly decreased in comparison to the ductal injection (−) group. The ductal injection of preservation solution increased the ATP level in the pancreas tissue and reduced trypsin activity during the digestion step. Annexin V and PI assays showed that the ductal injection prevents islet apoptosis. In a transplant model, the ductal injection improved islet graft function. These findings suggest that the ductal injection of preservation solution, especially the M-Kyoto solution, leads to improved outcomes for pancreatic islet transplantation. Based on these data, this technique is now used for clinical islet transplantation from non-heart-beating donor pancreata or living donor pancreas.

Keywords: Ductal injection; Islet isolation; Islet transplantation; M-Kyoto solution; UW solution

Document Type: Research Article

DOI: http://dx.doi.org/10.3727/000000008783907062

Affiliations: 1: Second Department of Surgery, Fujita Health University, Aichi 470-1192, Japan 2: Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan 3: Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan 4: Transplantation Unit, Kyoto University Hospital, Kyoto 606-8507, Japan 5: Baylor Institute for Immunology Research, Baylor Research Institute, Dallas, TX 75204, USA, Second Department of Surgery, Fujita Health University, Aichi 470-1192, Japan

Publication date: January 1, 2008

More about this publication?
  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.
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