Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function
Abstract:For islet transplantation, it is important to obtain an available islet mass adequate for diabetes reversal from a single donor pancreas. A recent report demonstrated that the use of M-Kyoto solution instead of UW solution improved islet yields in the two-layer method for pancreas preservation. The present study investigated whether the ductal injection of a large volume of preservation solution (UW and M-Kyoto solution) before pancreas storage improves islet yields. Islet yield both before and after purification was significantly higher in the ductal injection (+) group compared with the ductal injection (−) group. TUNEL-positive cells in the ductal injection (+) group were significantly decreased in comparison to the ductal injection (−) group. The ductal injection of preservation solution increased the ATP level in the pancreas tissue and reduced trypsin activity during the digestion step. Annexin V and PI assays showed that the ductal injection prevents islet apoptosis. In a transplant model, the ductal injection improved islet graft function. These findings suggest that the ductal injection of preservation solution, especially the M-Kyoto solution, leads to improved outcomes for pancreatic islet transplantation. Based on these data, this technique is now used for clinical islet transplantation from non-heart-beating donor pancreata or living donor pancreas.
Document Type: Research Article
Affiliations: 1: Second Department of Surgery, Fujita Health University, Aichi 470-1192, Japan 2: Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan 3: Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan 4: Transplantation Unit, Kyoto University Hospital, Kyoto 606-8507, Japan 5: Baylor Institute for Immunology Research, Baylor Research Institute, Dallas, TX 75204, USA, Second Department of Surgery, Fujita Health University, Aichi 470-1192, Japan
Publication date: January 1, 2008
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