Plasma as a Scaffold for Regeneration of Neural Precursor Cells After Transplantation Into Rats With Spinal Cord Injury

Authors: Takenaga, Mitsuko1; Ohta, Yuki1; Tokura, Yukie1; Hamaguchi, Akemi1; Suzuki, Noboru2; Nakamura, Masaya3; Okano, Hideyuki4; Igarashi, Rie1

Source: Cell Transplantation, Volume 16, Number 1, January 2007 , pp. 57-65(9)

Publisher: Cognizant Communication Corporation

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Abstract:

The present study investigated whether plasma could be useful as a scaffold for cell transplantation in rats with spinal cord injury (SCI). Transplantation of cells with plasma promoted the recovery of SCI-induced motor dysfunction. Immunohistochemical analysis revealed that the grafted cells had differentiated into the neural lineage. When dissociated neural precursor cells were cultured with plasma, extensive neurite outgrowth was observed along with increased expression of p35 and NF68. Neural markers were also expressed by the cultured cells. Culture with plasma reduced thymidine incorporation, but promoted cell growth and increased the RNA contents. These findings suggest that the cells underwent differentiation into neurons in the presence of plasma. In conclusion, plasma could be a promising scaffold for cell transplantation therapy.

Keywords: BBB score; Neural precursor cell; Plasma; Serum; Spinal cord injury

Document Type: Research Article

DOI: http://dx.doi.org/10.3727/000000007783464434

Affiliations: 1: Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki 216-8512, Japan 2: Department of Immunology and Medicine, St. Marianna University School of Medicine, Kawasaki 216-8512, Japan 3: Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan 4: Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan

Publication date: January 1, 2007

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  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.

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