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Functional MR Microimaging of Pancreatic -Cell Activation

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Abstract:

The increasing incidence of diabetes and the need to further understand its cellular basis has resulted in the development of new diagnostic and therapeutic techniques. Nonetheless, the quest to noninvasively ascertain -cell mass and function has not been achieved. Manganese (Mn)-enhanced MRI is presented here as a tool to image -cell functionality in cell culture and isolated islets. Similar to calcium, extracellular Mn was taken up by glucose-activated -cells resulting in 200% increase in MRI contrast enhancement, versus nonactivated cells. Similarly, glucose-activated islets showed an increase in MRI contrast up to 45%. Although glucose-stimulated Ca influx was depressed in the presence of 100 M Mn, no significant effect was seen at lower Mn concentrations. Moreover, islets exposed to Mn showed normal glucose sensitivity and insulin secretion. These results demonstrate a link between image contrast enhancement and -cell activation in vitro, and provide the basis for future noninvasive in vivo imaging of islet functionality and -cell mass.

Keywords: Activation; Islets; MRI; Manganese; -Cells

Document Type: Research Article

DOI: https://doi.org/10.3727/000000006783982151

Affiliations: 1: Department of Bioengineering, University of Illinois at Chicago, 851 S. Morgan St., Chicago, IL, 60607, USA, Division of Transplantation, Hospital, University of Illinois at Chicago, 840 S. Wood St., Chicago, IL, 60612, USA 2: Division of Transplantation, Hospital, University of Illinois at Chicago, 840 S. Wood St., Chicago, IL, 60612, USA 3: Department of Biomedical Engineering, Boston University, 44 Cummington St., Boston, MA, 20215, USA 4: Department of Medicine, Section of Endocrinology, University of Chicago, 5841 South Maryland Ave, Chicago, IL, 60637, USA 5: Department of Bioengineering, University of Illinois at Chicago, 851 S. Morgan St., Chicago, IL, 60607, USA

Publication date: 2006-02-01

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  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.
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