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Ameliorating Injury During Preservation and Isolation of Human Islets Using the Two-Layer Method With Perfluorocarbon and UW Solution

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Abstract:

This study assessed the effects of a two-layer method (TLM), using perfluorocarbon and UW solution, on the quality of human pancreata following storage and islet yield/function after isolation. In part A, TLM was applied immediately after procurement and the energetic profile was compared to a group treated with UW solution only (control) throughout 24-h storage. In part B, cadaveric human pancreata were procured and subjected to a TLM after cold storage in UW solution (TLM group) or UW solution (control group). Energetics, lipid peroxidation, and islet recovery/function were assessed after preservation at 4°C. In part A, after 9-h storage, the energetic profile (ATP, ATP/ADP, energy charge) for the TLM group was superior to controls. In part B, TLM treatment resulted in consistently greater ATP, ATP/ADP, and energy charge values than with storage in UW solution alone ( p < 0.05). UW treatment resulted in 40% greater peroxidative damage than in the TLM group ( p < 0.05). Islet recovery and functional viability were 30–40% higher following TLM treatment ( p < 0.05). These data support the hypothesis that islet viability and yields can be significantly improved using a brief period of TLM treatment following conventional UW storage; reduced energetic and oxidative stress are implicated as potential mechanisms.

Keywords: Islets; Perfluorocarbon; Preservation; Two-layer method; University of Wisconsin solution

Document Type: Research Article

DOI: http://dx.doi.org/10.3727/000000006783982070

Affiliations: 1: Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada 2: Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada, Clinical Islet Isolation Laboratory, University of Alberta Hospital/Capital Health Authority, Edmonton, Alberta, Canada 3: Clinical Islet Isolation Laboratory, University of Alberta Hospital/Capital Health Authority, Edmonton, Alberta, Canada 4: Department of Gastroenterological Surgery, Graduate School of Medicine, Kobe University, Kobe, Japan

Publication date: February 1, 2006

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  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.
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