Ameliorating Injury During Preservation and Isolation of Human Islets Using the Two-Layer Method With Perfluorocarbon and UW Solution
Abstract:This study assessed the effects of a two-layer method (TLM), using perfluorocarbon and UW solution, on the quality of human pancreata following storage and islet yield/function after isolation. In part A, TLM was applied immediately after procurement and the energetic profile was compared to a group treated with UW solution only (control) throughout 24-h storage. In part B, cadaveric human pancreata were procured and subjected to a TLM after cold storage in UW solution (TLM group) or UW solution (control group). Energetics, lipid peroxidation, and islet recovery/function were assessed after preservation at 4°C. In part A, after 9-h storage, the energetic profile (ATP, ATP/ADP, energy charge) for the TLM group was superior to controls. In part B, TLM treatment resulted in consistently greater ATP, ATP/ADP, and energy charge values than with storage in UW solution alone ( p < 0.05). UW treatment resulted in 40% greater peroxidative damage than in the TLM group ( p < 0.05). Islet recovery and functional viability were 30–40% higher following TLM treatment ( p < 0.05). These data support the hypothesis that islet viability and yields can be significantly improved using a brief period of TLM treatment following conventional UW storage; reduced energetic and oxidative stress are implicated as potential mechanisms.
Document Type: Research Article
Affiliations: 1: Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada 2: Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada, Clinical Islet Isolation Laboratory, University of Alberta Hospital/Capital Health Authority, Edmonton, Alberta, Canada 3: Clinical Islet Isolation Laboratory, University of Alberta Hospital/Capital Health Authority, Edmonton, Alberta, Canada 4: Department of Gastroenterological Surgery, Graduate School of Medicine, Kobe University, Kobe, Japan
Publication date: February 1, 2006
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