Subcutaneous Transplantation of Islets Into Streptozocin-Induced Diabetic Rats

Authors: Halberstadt, Craig R.1; Williams, Deana1; Emerich, Dwaine2; Goddard, Moses2; Vasconcellos, Alfred V.2; Curry, William1; Bhatia, Aashim1; Gores, Paul F.1

Source: Cell Transplantation, Volume 14, Number 8, August 2005 , pp. 595-605(11)

Publisher: Cognizant Communication Corporation

Buy & download fulltext article:

OR

Price: $79.00 plus tax (Refund Policy)

Abstract:

Pancreatic islet transplantation into type 1 diabetic patients is currently being performed by intraportal infusion. This method, albeit reproducible, has some disadvantages including potential development of portal hypertension, hemorrhage, and an inability to retrieve or detect the transplanted tissue. Other transplant sites have been examined in animal models including the omentum, peritoneal cavity, and the spleen. A transplant site that has not been successful in supporting functional islet tissue transplantation in humans is the subcutaneous space due primarily to the lack of a well-defined vascular bed. This site has many favorable characteristics such as ease of access for transplantation and potential for removal of the transplanted tissue with a minimally invasive surgical procedure. This report addresses the evaluation of a subcutaneously placed device for the support of rat syngeneic islet transplantation in a streptozocin-induced diabetic model. The data generated support the use of this device for islet engraftment. In addition, beta cell function in this device compared favorably with the function of islets transplanted to the renal subcapsular space as well as islets within the native pancreas.

Keywords: Diabetes; IVGTT; Islets; Rat; Subcutaneous transplant; Syngeneic

Document Type: Research Article

DOI: http://dx.doi.org/10.3727/000000005783982792

Affiliations: 1: Department of General Surgery, Carolinas Medical Center, Charlotte, NC 28232, USA 2: LCT BioPharma, Inc., Providence, RI, USA

Publication date: August 1, 2005

More about this publication?
  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.

Tools

Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page