Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells

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Abstract:

Mesenchymal stem cells (MSCs) have been successfully isolated from a broad range of adult, fetal, and other nonembryonic tissues. Fetal lung has been identified as a rich source of MSCs. However, the biological characteristics and differentiation potential of fetal lung MSCs remain to be explored. In this study, we established a series of methods for isolation and expansion of fetal lung MSCs. These MSCs could withstand more than 40 passages without obvious decline in proliferation ability, significant changes in morphology, and expression of cell markers. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD105, CD166, and HLA-ABC, but not CD14, CD31, CD34, CD38, CD41a, CD42b, CD45, CD49d, CD61, CD106, CD133, and HLA-DR. Cell cycle analysis revealed that when the MSCs reached their log phase of growth, more than 90% of the cells were in G0/G1 phase while the proportion of cells in S phase and G2/M phase were about 5.56% and 2.08% cells, respectively. These MSCs could differentiate into neural cells in addition to their mesenchymal differentiation potential. Our data suggest that the fetal lung MSC population is an alternative source of stem cells for cell-based therapy of neurological defects or mesenchymal-originating diseases.

Keywords: Bone marrow; Fetal lung; Mesenchymal stem cell; Neural differentiation; Stem cell plasticity

Document Type: Review Article

DOI: http://dx.doi.org/10.3727/000000005783983070

Affiliations: 1: National Research Center for Stem Cell Engineering & Technology,State Key Laboratory of Experimental Hematology, Institute of Hematology,Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of China, 2: Department of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China, 3: National Research Center for Stem Cell Engineering & Technology,State Key Laboratory of Experimental Hematology, Institute of Hematology,Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of China 4: Department of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China

Publication date: May 1, 2005

More about this publication?
  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.
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