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Brain Transplantation of Neural Stem Cells Cotransduced With Tyrosine Hydroxylase and GTP Cyclohydrolase 1 in Parkinsonian Rats

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Abstract:

Neural stem cells (NSCs) of the central nervous system (CNS) recently have attracted a great deal of interest not only because of their importance in basic research on neural development, but also in terms of their therapeutic potential in neurological diseases, such as Parkinson's disease (PD). To examine if genetically modified NSCs are a suitable source for the cell and gene therapy of PD, an immortalized mouse NSC line, C17.2, was transduced with tyrosine hydroxylase (TH) gene and with GTP cyclohydrolase 1 (GTPCH1) gene, which are important enzymes in dopamine biosynthesis. The expression of TH in transduced C17.2-THGC cells was confirmed by RT-PCR, Western blot analysis, and immunocytochemistry, and expression of GTPCH1 by RT-PCR. The level of L-DOPA released by C17.2-THGC cells, as determined by HPLC assay, was 3793 pmol/106 cells, which is 760-fold higher than that produced by C17.2-TH cells, indicating that GTPCH1 expression is important for L-DOPA production by transduced C17.2 cells. Following the implantation of C17.2-THGcC NSCs into the striata of parkinsonian rats, a marked improvement in amphetamine-induced turning behavior was observed in parkinsonian rats grafted with C17.2-THGC cells but not in the control rats grafted with C17.2 cells. These results indicate that genetically modified NSCs grafted into the brain of the parkinsonian rats are capable of survival, migration, and neuronal differentiation. Collectively, these results suggest that NSCs have great potential as a source of cells for cell therapy and an effective vehicle for therapeutic gene transfer in Parkinson's disease.

Keywords: Brain transplantation; C17.2 cell line; GTP cyclohydrolase 1; L-DOPA; Neural stem cell; Parkinson's disease; Tyrosine hydroxylase

Document Type: Review Article

DOI: http://dx.doi.org/10.3727/000000005783983133

Affiliations: 1: Department of Neurosurgery, Ajou University School of Medicine, Suwon, Korea, Department of Brain Disease Research Center, Ajou University School of Medicine, Suwon, Korea 2: Department of Brain Disease Research Center, Ajou University School of Medicine, Suwon, Korea 3: Department of Neurosurgery, Ajou University School of Medicine, Suwon, Korea 4: Burnham Institute, La Jolla, CA 5: Department of Brain Disease Research Center, Ajou University School of Medicine, Suwon, Korea, Division of Neurology, Department of Medicine, University of British Columbia, Vancouver, BC, Canada

Publication date: April 1, 2005

More about this publication?
  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.
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