Magnetic Resonance Tracking of Human CD34+ Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted Into Injured Rat Brain
Abstract:Magnetic resonance imaging (MRI) provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied whether superparamagnetic nanoparticles (CD34 microbeads), used clinically for specific magnetic sorting, can be used as a magnetic cell label for in vivo cell visualization. Human cells from peripheral blood were selected by CliniMACS+rm CD34 Selection Technology (Miltenyi). Purified CD34+ cells were implanted into rats with a cortical photochemical lesion, contralaterally to the lesion. Twenty-four hours after grafting, the implanted cells were detected in the contralateral hemisphere as a hypointense spot on T2 weighted images; the hypointensity of the implant decreased during the first week. At the lesion site we observed a hypointensive signal 10 days after grafting that persisted for the next 3 weeks, until the end of the experiment. Prussian blue and anti-human nuclei staining confirmed the presence of magnetically labeled human cells in the corpus callosum and in the lesion 4 weeks after grafting. CD34+ cells were also found in the subventricular zone (SVZ). Human DNA (a human-specific 850 base pair fragment of α-satellite DNA from human chromosome 17) was detected in brain tissue sections from the lesion using PCR, confirming the presence of human cells. Our results show that CD34 microbeads superparamagnetic nanoparticles can be used as a magnetic cell label for in vivo cell visualization. The fact that microbeads coated with different commercially available antibodies can bind to specific cell types opens extensive possibilities for cell tracking in vivo.
Document Type: Review Article
Affiliations: 1: Center for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic, MR-Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic 2: Institute of Experimental Medicine ASCR, Prague, Czech Republic 3: Institute of Experimental Medicine ASCR, Prague, Czech Republic, Center for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic 4: Institute of Hematology and Blood Transfusion, Prague, Czech Republic 5: Department of Paediatric Haematology and Oncology, Charles University, Second Medical Faculty, Prague, Czech Republic 6: Department of Pathology and Molecular Medicine, Charles University, Second Medical Faculty, Prague, Czech Republic 7: Center for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic, *Institute of Hematology and Blood Transfusion, Prague, Czech Republic
Publication date: April 1, 2005
- Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.