Maintenance of Functional Human Cancellous Bone and Human Hematopoiesis in NOD/SCID Mice
Attempts were made to establish models to study interactions between marrow stromal cells and hematopoietic cells in vivo. The approach was to create a NOD-SCID-hu murine model of long-term human hematopoiesis by implantation of a human adult bone fragment. Nine to 12 weeks posttransplantation,
human CD45+ cells were detected in the blood and the spleen of some mice. The histology of the human transplant showed that human bone fragment was viable at 9 weeks. Moreover, vessels of human origin, as assessed by immunohistochemical detection of human β2-microglobulin,
were observed in the mouse tissue surrounding the transplanted human fragment.
Keywords: Hematopoiesis; Human bone fragment; In vivo model; NOD/SCID mice
Document Type: Research Article
Affiliations: 1: *Department of Cytology and Histology University of Liège, Liège, 4000, Belgium 2: †Department of Pathological Anatomy, University of Liège, Liège, 4000, Belgium
Publication date: 01 January 2004
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