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BOOK REVIEW Hematopoietic Stem Cell ProtocolChristopher A. Klug, Craig T. Jordan (Clifton, NJ: Humana Press, 2002; ISBN 0-89603-812-2)

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“(Stem cells) are an issue that is one of the most profound of our time,” said George W. Bush at his inaugural address. In recent years, the field of stem cells has become one of the hottest and most rapidly growing areas in biological and medical sciences. Stem cells have become the focus of intense interest from a growing, multidisciplinary community that now has new investigative tools to isolate and characterize these elusive cell types. Hematopoietic stem cells (HSCs), which are studied in the earliest and most extensive phase, have become one of the hallmarks of stem cell research. Furthermore, the study of embryonic stem cells in recent years has also influenced the study of HSCs, and vice versa. In the literature of HSCs, many methods and protocols have been reported with different successes, which is perhaps why there has not been a single book that describes a full range of methods collectively. Christopher A. Klug and Craig T. Jordan, leading stem cell investigators, along with 40 collaborators, have edited key laboratory methods in a book entitled Hematopoietic Stem Cell Protocol, giving us a comprehensive and step-by-step description of the methods for investigating HSCs from mouse and human origin, both embryonic and adult. It includes a series of protocols from isolation, characterization, culture, cloning, cell expansion, genetic modification by retroviral transduction, and identification of genetic expression by RT-PCR and protein expression by 2D gene expression fingerprinting, as well as mouse animal models for in vivo assays. It is a full description of modern cellular and molecular techniques well adapted to the experiments in HSCs. As the editors said in the preface of this book, “The ability to highly purify and characterize HSCs from mice and humans has opened up an exceedingly rich field of basic science research with enormous clinical potential. Many of the techniques used in studies of HSC biology have become more standardized over the last several years, which makes it possible to compile a set of methods that can be used by both seasoned investigators and novices in the stem cell field. We have attempted to be as comprehensive as possible and yet focus on what we perceive to be the most widely used approaches for studies of murine and human HSC.”
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Document Type: Book Review

Affiliations: 1: *Center of Excellence for Aging and Brain Repair, University of South Florida College of Medicine, Tampa, FL 2: †Saneron CCEL Therapeutics, Inc., Tampa, FL 3: ‡Cryobanks International, Inc., Alamonte Springs, FL

Publication date: 2004-01-01

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  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.

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