Mobilized Peripheral Blood Cells Administered Intravenously Produce Functional Recovery in Stroke

Authors: Willing A.E.¹; Vendrame M.¹; Mallery J.¹; Cassady C.J.¹; Davis C.D.²; Sanchez-Ramos J.¹; Sanberg P.R.¹

Source: Cell Transplantation, Volume 12, Number 4, 2003 , pp. 449-454(6)

Publisher: Cognizant Communication Corporation

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Abstract:

Filgratism (granulocyte colony stimulating factor, G-CSF)-mobilized peripheral blood progenitor cells (PBPCs) have replaced bone marrow (BM) as a preferred source of autologous stem cells, in light of the faster hematologic recovery and lesser supportive care requirement exhibited by PBPC transplants. Other hematopoietic stem cells, like the human umbilical cord blood-derived stem cells (hUCBs), and nonhematopoietic stem cells have been shown to improve motor function in rodent models of injury and degenerative disease. In the present study we transplanted either G-CSF-mobilized PBPCs or hUCBs in rats 24 h after permanent middle cerebral artery occlusion (MCAO), and assessed their behavioral abnormalities in spontaneous activity and spontaneous motor asymmetry. In both transplanted groups of rats we observed a significant reduction of the stroke-induced hyperactivity compared with nontransplanted, stroked animals. In addition, transplantation of G-CSF PBPC and hUCB cells prevented the development of extensive motor asymmetry. Our findings raise the possibility that PBPCs could provide a novel transplantation therapy to treat stroke.

Keywords: Peripheral blood; Stem cells; Filgrastim; G-CSF; Stroke; Middle cerebral artery occlusion; Behavioral analysis

Document Type: Research article

Affiliations: 1: *Center of Excellence for Aging & Brain Repair, University of South Florida College of Medicine, 12901 Bruce B. Downs Blvd, Tampa, FL 33612 2: ††Saneron CCEL Therapeutics, Inc., 13101 Telecom Park, Suite 105, Tampa FL 33617

Publication date: 2003-01-01

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Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.

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Mobilized Peripheral Blood Cells Administered Intravenously Produce Functional Recovery in Stroke

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