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Intracerebral Transplantation and Successful Integration of Astrocytes Following Genetic Modification With a High-Capacity Adenoviral Vector

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To investigate the ability of genetically modified astrocytes to integrate into adult rat brain, two spontaneously immortalized cell lines and the allogenic nontumorigenic glioma cell line F98 were transduced with a high-capacity adenoviral vector (HC-Adv) expressing the EGFP gene from the hCMV promoter. In organotypic slice cultures the transduced astrocytes were shown to integrate into the brain tissue. Following transplantation of the transduced astrocytes into the striatum of adult rats, the transplanted cells survived at least for 6 weeks, continuously expressed the EGFP transgene, in close neighborhood with cells of the recipient tissue executing their differentiation capacity along the glial lineage. Thus, HC-Adv transduced astrocytes are promising vehicles to locally deliver therapeutic proteins for the treatment of neurodegenerative diseases.
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Keywords: Key words: Intracerebral transplantation; Neurotrophic factors; Parkinson's disease; Astrocytes; High-capacity adenoviral vector

Document Type: Research Article

Affiliations: 1: *Department of Anatomy I, University of Cologne, Joseph-Stelzmann Str. 9, 50931 Köln, Germany 2: †Center for Molecular Medicine (ZMMK), University of Cologne, Joseph-Stelzmann Str. 9, 50931 Köln, Germany 3: ‡Clinic for Stereotaxy and Functional Neurosurgery, University of Cologne, Joseph-Stelzmann Str. 9, 50931 Köln, Germany

Publication date: 2002-01-01

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  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.

    Cell Transplantation is now being published by SAGE. Please visit their website for the most recent issues.

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