@article {Jahr:2002:0963-6897:513,
	author = "Jahr H. and Huszmann B. and Eckhardt T. and Bretzel R.G.",
	title = "Successful Single Donor Islet Allotransplantation in the Streptozotocin Diabetes Rat Model",
	journal = "Cell Transplantation",
	volume = "11",
	number = "6",
	year = "2002",
	abstract = "<P>The objective of this study was to define pretransplant islet culture conditions for optimum tissue engraftment in the rat islet allotransplantation model. Lewis rat islets were cultured in TCM 199/5% fetal calf serum for 1 day at 37&#176;C, followed by 1 day of culture at 22&#176;C. When islets from single donors were allotransplanted intraportally into single streptozotocin-diabetic Wistar-Furth rats, complete normoglycemia was restored within 1 day after transplantation in seven out of seven rats, and persisted up to immunological rejection about 1 week later. In five out of six rats receiving a posttransplant injection of antilymphocyte serum, plasma glucose was normalized for &#062;100 days. These data demonstrate, for the first time, successful single-donor-to-single-recipient transplantation of allogeneic rat pancreatic islets. Because islet engraftment may still be regarded as a main problem for clinical islet transplantation, the pretransplant islet culture regimen outlined in this article may lead to a more efficient use of donor pancreatic islet tissue in the clinical setting, as well.</P>",
	pages = "513-518",
	url = "http://www.ingentaconnect.com/content/cog/ct/2002/00000011/00000006/ct334",
	keyword = "Key words: Islet allotransplantation; Single donor"
}