Formation of Human Fibroblast Aggregates (Spheroids) by Rotational Culture
Source: Cell Transplantation, Volume 10, Numbers 4-5, 2001 , pp. 441-445(5)
Publisher: Cognizant Communication Corporation
Abstract:In the current study, we attempted to form aggregates of fibroblasts by rotationally shaking, declining fibroblast–material interactions, and augmenting cell–cell interactions. In addition, to promote cell–cell interactions, the medium was supplemented with insulin, dexamethasone, and basic fibroblast growth. Under such improved culture conditions, normal neonatal human dermal fibroblasts formed spheroidal aggregates within 1 day of rotation on a rotational shaker. The aggregates that formed had irregular shapes and were composed from only several cells after 12 h. However, they became nearly spheroidal after 24 h of shaking. The aggregates were approximately 240 μm in diameter. After 36 h of shaking, their shape became more rounded and their surfaces became smoother. No evidence of necrosis in the center of the aggregates was observed, although a small number of dead cells was scattered throughout the aggregates. After 24–36 h, aggregates of normal human fibroblasts were collected and reinoculated onto a scaffold composed of polyglycolic acid, which is used commercially as a scaffold for artificial skin, coated with collagen. The aggregates were successfully trapped to the mesh of polyglycolic acid and became attached within 24 h. Therefore, the aggregates could provide an alternative method for seeding fibroblasts to scaffold for an artificial skin, such as a mesh of polyglycolic acid.
Document Type: Research Article
Affiliations: 1: †CREST, Japan Science and Technology Corporation, Japan 2: ‡Dimensional Cell & Tissue-Engineering Group, National Institute for Interdisciplinary Research, Agency of Industrial Science and Technology, Ministry of International Trade and Industry, Tsukuba, Ibaraki, Japan 3: §Institute of Industrial Science, University of Tokyo, Minato-ku, Tokyo, Japan 4: ¶National Institute for Research in Inorganic Materials, Japan
Publication date: 2001-01-01
- Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow work to be disseminated to a wider audience and also entitle the corresponding author to a free PDF, as well as prepublication of an unedited version of the manuscript.