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Probing Enhanced Cytochrome P450 2B1/2 Activity in Rat Hepatocyte Spheroids Through Confocal Laser Scanning Microscopy

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Cytochrome P450 (CYP450) enzymes are essential for xenobiotic metabolism. Although CYP450s are found in many tissues, CYP2B1/2 are primarily expressed in the rat liver. The constitutive expression in vivo of CYP2B1/2 is low but it is induced in the presence of various drugs such as phenobarbital (PB). In this study, CYP2B1/2 activity in cultured hepatocytes was assessed in situ with the introduction of a fluorogenic substrate, pentoxyresorufin. The product of 7-pentoxyresorufin-O-dealkylation (PROD), which is catalyzed specifically by CYP2B1/2, was detected using confocal laser scanning microscopy (CLSM). Primary hepatocytes cultured as monolayers on collagen-coated surfaces exhibited background PROD activity and minimal PB inducibility after 4 days in culture. In contrast, rat hepatocytes organized in compacted aggregates, or spheroids, exhibited higher levels of PROD activity and retained their ability for PB induction. The results from the CLSM analysis were verified by RT-PCR and Western immunoblotting analysis. Furthermore, CLSM in conjunction with image processing techniques and three-dimensional reconstruction revealed the localization of enhanced PROD activity in the center of spheroids. The results support the use of CLSM as a powerful tool for investigating CYP2B1/2 activity in cultured rat hepatocytes.

Keywords: CLSM;; Key words: Hepatocytes; PROD; Self-assembly

Document Type: Research Article


Affiliations: 1: *Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455-0132 2: †Department of Applied Life Science, Sojo University, Ikeda, Kumamoto 860-0082, Japan 3: ‡Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455-0132

Publication date: March 1, 2001

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