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Open Access Cryopreservation of Induced Pluripotent Stem Cells

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Abstract:

Induced pluripotent stem (iPS) cells have attracted attention as a promising cell source for medical treatment that could replace marrow stromal cells (MSCs) and adipose tissue-derived stem cells (ASCs). These pluripotent cells can be induced in vitro and in vivo to differentiate into various tissues and organs. The cells will be useful for regenerative medicine, cell therapy, and drug screening. Vitrification is used, as well as a rapid-freeze method, for colony-forming iPS cells. However, the method requires a high degree of technical skill. We herein report a more convenient method for freezing iPS cells in suspension. We examined the proliferation potency of cryopreserved mouse iPS cells using culture medium, 10% DMSO, 10% glycerol, 5% DMSO, 5% glycerol, 5% DMSO + 5% glycerol, cell-freezing medium-DMSO, cell-freezing medium-glycerol, Cell Banker 1, Cell Banker 1+, Cell Banker 2, and Cell Banker 3 as cryopreservation solutions. Among them, Cell Banker 3 showed the highest efficacy in terms of the proliferation of mouse iPS cells. The mouse iPS cells cryopreserved in Cell Banker 3 at −80°C for 12 months maintained a high proliferation rate and an undifferentiated status. The formation of teratomas was also examined. In conclusion, Cell Banker 3 allows for freezing of iPS cells in suspension.

Keywords: Cryopreservation; Induced pluripotent stem (iPS) cells; Pluripotency; Slow freezing

Document Type: Research Article

DOI: http://dx.doi.org/10.3727/215517912X639405

Publication date: January 1, 2012

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  • The importance of translating original, peer-reviewed research and review articles on the subject of cell therapy and its application to human diseases to society has led to the formation of the journal Cell Medicine. To ensure high-quality contributions from all areas of transplantation, the same rigorous peer review will be applied to articles published in Cell Medicine. Articles may deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, and stem cells, among others. Basic clinical studies and immunological research papers may also be featured if they have a translational interest. To provide complete coverage of this revolutionary field, Cell Medicine will report on relevant technological advances and their potential for translational medicine. Cell Medicine will be a purely online Open Access journal. There will therefore be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow your work to be disseminated to a wider audience and also entitle you to a free PDF, as well as prepublication of an unedited version of your manuscript.
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