Authors: Noguchi, Hirofumi; Naziruddin, Bashoo; Shimoda, Masayuki; Chujo, Daisuke; Takita, Morihito; Sugimoto, Koji; Itoh, Takeshi; Onaca, Nicholas; Levy, Marlon F.; Matsumoto, Shinichi

Source: Cell Medicine, Volume 3, Numbers 1-3, 2012 , pp. 33-41(9)

Publisher: Cognizant Communication Corporation

Abstract:

For islet transplantation, islet purification minimizes the risks associated with islet infusion through the portal vein. However, islet purification may result in decreased numbers of islets recovered from digested tissue. In this study, we evaluated the effectiveness of performing supplemental purification (SP) after regular purification (RP). We designed the densities of low- and high-density solutions based on the outcome of RP. Moreover, a combined continuous osmolality/continuous density gradient for the SP was used in this study. Low-density/osmolality (1.075-1.110 g/cm³/400-410 mOsm/kg) and high-density/osmolality (1.090-1.125 g/cm³/495-505 mOsm/kg) solutions were produced by changing the volumetric ratio of iodixanol, 10 × HBSS, and RP solutions. The percentage of islet recovery (postpurification IE/prepurification IE × 100) after RP was 77.3 ± 5.6%, and the percentage of islet recovery after addition of SP was 85.3 ± 5.4%. In vitro and in vivo assessments showed that islet viability and function were not altered by the additional purification step. These data suggest that the addition of SP could contribute approximately 8% to islet recovery with viability and potency comparable to that obtained by RP and, therefore, that usage of the combined continuous density and continuous osmolality gradient for SP could efficiently improve islet equivalents in the final preparation.

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Keywords: Islet transplantation; Islet isolation; Supplemental purification; Regular purification; Osmolality; Density

Document Type: Research article

DOI: http://dx.doi.org/10.3727/215517912X639388

Publication date: 2012-01-01

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• The importance of translating original, peer-reviewed research and review articles on the subject of cell therapy and its application to human diseases to society has led to the formation of the journal Cell Medicine. To ensure high-quality contributions from all areas of transplantation, the same rigorous peer review will be applied to articles published in Cell Medicine. Articles may deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, and stem cells, among others. Basic clinical studies and immunological research papers may also be featured if they have a translational interest. To provide complete coverage of this revolutionary field, Cell Medicine will report on relevant technological advances and their potential for translational medicine. Cell Medicine will be a purely online Open Access journal. There will therefore be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. This will allow your work to be disseminated to a wider audience and also entitle you to a free PDF, as well as prepublication of an unedited version of your manuscript.

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• In this Subject: Anatomy & Physiology ,  Biology ,  Surgery
• By this author: Noguchi, Hirofumi ;  Naziruddin, Bashoo ;  Shimoda, Masayuki ;  Chujo, Daisuke ;  Takita, Morihito ;  Sugimoto, Koji ;  Itoh, Takeshi ;  Onaca, Nicholas ;  Levy, Marlon F. ;  Matsumoto, Shinichi

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