Authors: VOLENBERG, D S¹; TRANEL, P J¹; HOLT, J F¹; SIMMONS, F W¹; WELLER, S C²; SHARKHUU, A²; RIECHERS, D E¹

Source: Weed Research, Volume 47, Number 4, August 2007 , pp. 353-363(11)

Publisher: Wiley-Blackwell

Abstract:

Summary

Glasshouse and laboratory experiments were conducted on acetolactate synthase (ALS) homozygous resistant Solanum ptycanthum biotypes from Illinois (IL-R) and Indiana (IN-R), and homozygous susceptible biotypes from Illinois (IL-S) and Indiana (IN-S). Genetic similarity of biotypes was assessed by random amplified polymorphic DNA (RAPD) markers, which determined that the Illinois biotypes are more similar to each other than to the IN-R biotype. ALS enzyme activity from the IL-R and IN-R biotypes had I₅₀ values of 362 and 352 μM imazamox respectively. Dose-response experiments using three- to four-leaf-stage plants of the IL-R and IN-R biotypes had GR₅₀ values of 242 and 69 g ae ha⁻¹ imazamox respectively. Whole-plant and ALS enzyme results are different than previously reported values in the literature, which was attributed in the current study to the original IN-R population having individuals that were segregating for ALS resistance. Metabolism studies showed no difference in percentage [¹⁴C]imazamox remaining between the IL-R and IN-R biotypes up to 72 h after treatment. The IL-S biotype metabolised [¹⁴C]imazamox approximately two times faster than the IL-R and IN-R biotypes and this trait was heritable. Response of F₃ plants containing homozygous ALS-resistant alleles from the IL-R biotype in a genetic background of 50% Illinois and 50% Indiana biotypes suggests that genetic factors other than an altered target site or metabolism may also contribute to the magnitude of resistance at the whole-plant level in resistant biotypes.

Keywords: acetolactate synthase; eastern black nightshade; imidazolinone; metabolism; RAPD

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-3180.2007.00563.x

Affiliations: 1: Department of Crop Science, University of Illinois, Urbana, IL, USA 2: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, USA

Publication date: 2007-08-01

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