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In Vitro Efficacy of a Polyhexamethylene Biguanide-Impregnated Gauze Dressing Against Bacteria Found in Veterinary Patients

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To evaluate the in vitro efficacy of polyhexamethylene biguanide (PHMB)-impregnated gauze dressing in limiting the growth of bacteria both within and underneath the dressing. Study Design

In vitro study. Methods

Squares of PHMB-impregnated and control gauze were placed on agar plates inoculated with 1 of 11 bacterial species, including 8 multi-resistant organisms. Growth under the gauze was assessed qualitatively after 24-hour incubation. Repeated use of sponges was used to evaluate residual inhibitory activity against Micrococcus lutea and Staphylococcus schleiferi ss. schleiferi. In a second procedure, PHMB-impregnated and control gauze squares were placed in sterile plastic wells and inoculated with 1 of 5 bacterial species, including Pseudomonas spp. and Klebsiella spp. Inhibition of bacterial growth within and underneath the dressing after 24-hour incubation was evaluated by quantifying the numbers of bacteria on the well floor and within each square. Results

PHMB-impregnated gauze provided greater inhibition of growth of 4/4 Gram-positive species and 2/6 Gram-negative species on inoculated plates compared with control gauze. Residual inhibitory activity of PHMB-impregnated gauze was significantly greater against M. lutea on all days and against S. schleiferi ss. schleiferi on days 1 and 4 compared with control. No bacteria were recovered from inoculated PHMB-impregnated gauze squares placed in sterile wells or from the well floor underneath. More than 9 × 105 colony-forming units (CFU) were recovered from inoculated control samples placed in sterile wells and more than 8.4 × 104 CFU were recovered from control well floors. Conclusion

PHMB-impregnated gauze dressing, when placed on inoculated agar plates, reduces growth of underlying bacteria, particularly Gram-positive species. Wet-inoculated PHMB-impregnated dressing prevents growth of Gram-positive and Gram-negative bacteria both within and underneath the dressing. Clinical Relevance

PHMB-impregnated dressings may be useful for reducing contamination of underlying wounds by bacterial pathogens.

Keywords: bacterial inhibition; gauze dressing; polyhexamethylene biguanide

Document Type: Research Article


Affiliations: From the Departments of Small Animal Clinical Sciences, Comparative Medicine, and Large Animal Clinical Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN

Publication date: July 1, 2004

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