Intraosseous Gentamicin Perfusion of the Distal Metacarpus in Standing Horses
To report tissue gentamicin concentrations after intraosseous (IO) perfusion in standing horses. Study Design––
In vivo study. Animals or Sample Population––
Twelve horses. Methods
Sedated horses had a cannulated cortical bone screw inserted into the dorsolateral aspect of the treated metacarpus and a tourniquet applied proximally. Gentamicin (2.2 mg/kg) diluted in sterile saline solution (0.1 mL/kg) was infused through the screw. Two horses were euthanatized at each time interval: 0, 2, 6, 12, 24, and 36 hours. Synovial fluid and bone samples were collected distal to the screw from both forelimbs. Gentamicin concentrations were measured using fluorescence polarization immunoassay. Results
The highest synovial fluid gentamicin concentrations were 385±273 g/mL (mean±SD) in the metacarpophalangeal joint, 225±205 g/mL in the proximal interphalangeal joint, 215±205 g/mL in the distal interphalangeal joint, 382±195 g/mL in the digital flexor tendon sheath, and 206±161 g/mL in the navicular bursa. The highest bone concentrations of gentamicin were 55±30 g/g in the distal metacarpus, 34±27 g/g in the proximal, 16±15 g/g in the middle, and 16±2.2 g/g in the distal phalanges, and 27±17 g/g in the proximal and 24±11 g/g in the distal sesamoid bones. Conclusion
Standing IO perfusion of gentamicin resulted in local antibiotic concentrations in the synovial structures and bones of the distal aspect of the limb that exceed the reported minimum inhibitory concentration of pathogens commonly implicated in equine orthopedic infections. Clinical Relevance
Standing IO perfusion of gentamicin in the distal aspect of the limb should be considered for treatment of orthopedic infections of this region in horses.
Document Type: Research Article
Affiliations: From the Departments of Clinical Studies and Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Canada.
Publication date: March 1, 2004