IngentaConnect Dengue virus antibodies in blood donors from an endemic area

Authors: Rodríguez Rodríguez, D.¹; Garza Rodríguez, M.¹; Chavarria, A. M.¹; Ramos-Jiménez, J.²; Rivera, M. A.³; Taméz, R. C.³; Farfan-Ale, J.⁴; Rivas-Estilla, A. M.

Source: Transfusion Medicine, Volume 19, Number 3, June 2009 , pp. 125-131(7)

Publisher: Blackwell Publishing

Abstract:

summary.

We evaluated the incidence of anti-Dengue virus (DENV) antibodies and dengue viremia in a region of Mexico with a high prevalence of dengue. DENV is the most important arthropod-borne virus in terms of human morbidity and mortality in America We tested 800 blood donors from a tertiary care teaching hospital that provides care in Northeast Mexico, to identify anti-DENV IgM and IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and DENV genome by reverse transcription polymerase chain reaction (RT-PCR). In addition, routine tests for donors including Brucella, Hepatitis C virus (HCV), Venereal Disease Research Laboratory (VDRL), HIV-1 and HBsAg identification were performed. We found that 59% of donors were reactive for anti-DENV IgG and none of them had reported recent DENV infection; however, 16 (2%) were reactive for anti-DENV IgM antibodies. None of them were viremic at the time of donation. Routine tests showed that the prevalence of anti-Brucella was 0.71%, anti-HCV 0.71%, anti-HIV-1-2 0.14%, HBsAg 0.14% and VDRL test 0.57%. Although DENV transmission by blood transfusion had not been confirmed in Mexico, the finding of a high prevalence of anti-DENV IgM-positive donors with asymptomatic manifestations and the recent viremia reported in blood donors suggests that this route of transmission might be possible.

Keywords: blood donors; blood transfusion; dengue antibodies; dengue fever; Dengue virus

Document Type: Research article

DOI: 10.1111/j.1365-3148.2009.00922.x

Affiliations: 1: Laboratory of Molecular Infectology, Department of Biochemistry and Molecular Medicine 2: Infectious Diseases Service, Department of Internal Medicine 3: Blood Transfusion Center, Department of Clinical Pathology, from School of Medicine and Hospital Universitario `Dr. Jose E. Gonzalez', Universidad Autónoma de Nuevo León Monterrey, NL, Mexico 4: Laboratory of Arbovirology, Department of Infectious and Vector Transmitted Diseases. Centro de Investigaciones Regionales “Hideyo Noguchi”, Universidad Autónoma de Yucatán

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