Authors: Kojima, Hisae¹; Suzuki, Takamasa¹; Kato, Takenori¹; Enomoto, Ken-ichi¹; Sato, Shusei²; Kato, Tomohiko²; Tabata, Satoshi²; Sáez-Vasquez, Julio³; Echeverría, Manuel³; Nakagawa, Tsuyoshi⁴; Ishiguro, Sumie¹; Nakamura, Kenzo

Source: The Plant Journal, Volume 49, Number 6, March 2007 , pp. 1053-1063(11)

Publisher: Wiley-Blackwell

Abstract:

Summary

Animal and yeast nucleolin function as global regulators of ribosome synthesis, and their expression is tightly linked to cell proliferation. Although Arabidopsis contains two genes for nucleolin, AtNuc-L1 is the predominant if not only form of the protein found in most tissues, and GFP-AtNuc-L1 fusion proteins were targeted to the nucleolus. Expression of AtNuc-L1 was strongly induced by sucrose or glucose but not by non-metabolizable mannitol or 2-deoxyglucose. Sucrose also caused enhanced expression of genes for subunits of C/D and H/ACA small nucleolar ribonucleoproteins, as well as a large number of genes for ribosomal proteins (RPs), suggesting that carbohydrate availability regulates de novo ribosome synthesis. In sugar-starved cells, induction of AtNuc-L1 occurred with 10 mmglucose, which seemed to be a prerequisite for resumption of growth. Disruption of AtNuc-L1 caused an increased steady-state level of pre-rRNA relative to mature 25S rRNA, and resulted in various phenotypes that overlap those reported for several RP gene mutants, including a reduced growth rate, prolonged lifetime, bushy growth, pointed leaf, and defective vascular patterns and pod development. These results suggest that the rate of ribosome synthesis in the meristem has a strong impact not only on the growth but also the structure of plants. The AtNuc-L1 disruptant exhibited significantly reduced sugar-induced expression of RP genes, suggesting that AtNuc-L1 is involved in the sugar-inducible expression of RP genes.

Keywords: nucleolin; ribosome synthesis; ribosomal protein genes; snoRNP; sugar

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-313X.2006.03016.x

Affiliations: 1: Laboratory of Biochemistry, Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan, 2: Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan, 3: Laboratoire Génome et Développement des Plantes, UMR CNRS-IRD 5096, Université de Perpignan, 66860 Perpignan Cedex, France, and 4: Research Institute of Molecular Genetics, Shimane University, Matsue, Shimane 690-8504, Japan

Publication date: 2007-03-01

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• By this author: Kojima, Hisae ;  Suzuki, Takamasa ;  Kato, Takenori ;  Enomoto, Ken-ichi ;  Sato, Shusei ;  Kato, Tomohiko ;  Tabata, Satoshi ;  Sáez-Vasquez, Julio ;  Echeverría, Manuel ;  Nakagawa, Tsuyoshi ;  Ishiguro, Sumie ;  Nakamura, Kenzo

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