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Free Content Identification of lymphogranuloma venereum‐associated Chlamydia trachomatis serovars by fluorescence in situ hybridisation – a proof‐of‐principle analysis

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Abstract:

We describe a proof‐of‐principle evaluation of a fluorescence in situ hybridisation (FISH) procedure to identify Chlamydia trachomatis serovars L1–L3, the causative agents of lymphogranuloma venereum, in cell cultures based on newly designed DNA probes. Rapid and easy‐to‐perform FISH could facilitate the diagnosis of lymphogranuloma venereum without nucleic acid amplification or serotyping, but requires broader evaluation studies, for example, in tropical high‐endemicity regions.

Keywords: Chlamydia trachomatis; cell culture; diagnostic microbiology; fluorescence in situ hybridisation; lymphogranuloma venereum

Document Type: Research Article

DOI: https://doi.org/10.1111/tmi.12271

Publication date: 2014-04-01

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