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Free Content Genetic variability in Plasmodium vivax aldolase gene in Korean isolates and the sensitivity of the Binax Now malaria test

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Summary Introduction of rapid malaria diagnostic tests (RDT) initiated numerous field evaluations in various epidemiologic settings. But the efficiency of some RTD kits based on aldolase raised reservations for direct implementation of RDT into clinical settings. We performed Binax Now malaria test in 84 Korean isolates and compared it with the traditional Giemsa stain microscopy test as the reference standard. The sensitivity of Binax Now was 62.0% for cases (52/84, 95% CI 51.2–71.6%) with 100.0% specificity (50/50, 95% confidence interval 92.9–100%). After the aldolase gene sequence analysis of 84 isolates, two synonymous mutations in aldolase gene were identified in both Binax Now positive and negative samples. No significant association between the mutations and Binax Now malaria tests was found. Thus, the genetic variability would not explain the poor performance of RDTs by detecting aldolase in ROK isolates.

French
L’introduction de nouveaux tests de diagnostic rapide de la malaria (TDR) a initié de nombreuses évaluations sur le terrain dans différents contextes épidémiologiques. Mais, l’efficacité de certains kits TDR basés sur l’aldolase a soulevé des réserves pour l’implémentation directe des TDR en milieu clinique. Nous avons appliqué le test Binax Now sur 84 isolats coréens de P. vivax et l’avons comparé au test traditionnel de la microscopie à la coloration Giemsa comme référence. La sensibilité de Binax Now était de 62,0% pour les cas de P. vivax (52/84, IC95%: 51,2%–71,6%) avec une spécificité de 100,0% (50/50, IC95%: 92,9%–100%). L’analyse de la séquence du gène aldolase des 84 isolats a révélé deux mutations synonymes à la fois dans les échantillons Binax Now positifs et négatifs. Aucune association significative n’été trouvée entre les mutations et le test Binax Now malaria. Dès lors, la variabilité génétique n’expliquerait pas la mauvaise performance des TDR pour P. vivax dans la détection de l’aldolase dans les isolats de la République de Corée.
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Keywords: Aldolasa; Binax Now; Especificidad; OptiMAL; Plasmodium vivax; Sensibilidad; aldolase; sensibilité; sensitivity; specificity; spécificité

Document Type: Research Article

Affiliations: 1:  Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul, Korea 2:  Department of Parasitology, Kangwon National University College of Medicine, Chuncheon, Korea 3:  Department of Malaria and Parasite Disease, National Institute of Health, KCDC, Seoul, Korea 4:  Department of Cellular Oncology, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul, Korea 5:  College of Bionano Technology, Gachon Bionano Research Institute, Kyungwon University, Seongnam-si, Kyeonggi Do, Korea

Publication date: 2011-02-01

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