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Free Content Evaluation of two commercially available ELISAs for the diagnosis of Japanese encephalitis applied to field samples

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Summary Objective  To compare two commercially available kits, Japanese Encephalitis-Dengue IgM Combo ELISA (Panbio Diagnostics) and JEV-CheX IgM capture ELISA (XCyton Diagnostics Limited), to a reference standard (Universiti Malaysia Sarawak – Venture Technologies VT ELISA). Methods  Samples were obtained from 172/192 children presenting to a site in rural India with acute encephalitis syndrome. Results  Using the reference VT ELISA, infection with Japanese encephalitis virus (JEV) was confirmed in 44 (26%) patients, with central nervous system infection confirmed in 27 of these; seven patients were dengue seropositive. Of the 121 remaining patients, 37 (31%) were JEV negative and 84 (69%) were JEV unknown because timing of the last sample tested was <10 day of illness or unknown. For patient classification with XCyton, using cerebrospinal fluid alone (the recommended sample), sensitivity was 77.8% (59.2–89.4) with specificity of 97.3% (90.6–99.2). For Panbio ELISA, using serum alone (the recommended sample), sensitivity was 72.5% (57.2–83.9) with specificity of 97.5% (92.8–99.1). Using all available samples for patient classification, sensitivity and specificity were 63.6% (95% CI: 48.9–76.2) and 98.4% (94.5–99.6), respectively, for XCyton ELISA and 75.0% (59.3–85.4) and 97.7% (93.3–99.2) for Panbio ELISA. Conclusion  The two commercially available ELISAs had reasonable sensitivities and excellent specificities for diagnosing JEV.


Comparer deux kits disponibles dans le commerce: ELISA encéphalite japonaise/dengue IgM combo (Diagnostics Panbio) et ELISA capture JEV-Chex IgM (Diagnostics XCyton Limited) à un étalon de référence (Universiti Malaysia Sarawak-Venture Technologies VT ELISA). Méthodes: 

Des échantillons ont été obtenus de 172/192 enfants présentant le syndrome encéphalitique aigu (SEA) dans un site rural en Inde. Résultats: 

En utilisation la référence VT ELISA, l’infection par le virus de l’EJ (JEV) a été confirmée chez 44 (26%) patients avec infection confirmée du SNC pour 27 d’entre eux. 7 patients étaient séropositifs pour la dengue. Sur les 121 patients restants, 37 (31%) étaient négatifs pour JEV et 84 (69%) n’ont pas eu de résultat JEV car la période de test du dernier échantillon était < 10 jours de maladie ou inconnue. Pour la classification des patients avec XCyton, en utilisant le LCR (échantillon recommandé) seul, la sensibilitéétait de 77,8% (59,2-89,4) et la spécificité de 97,3 (90,6-99,2). Pour ELISA Panbio, en utilisant le sérum seul (échantillon recommandé), la sensibilitéétait de 72,5 (57,2-83,9) et la spécificité de 97,5% (92,8-99,1). En utilisant tous les échantillons disponibles pour la classification des patients, la sensibilité et la spécificitéétaient de 63,6% (IC95%: 48,9-76,2) et 98,4% (94,5-99,6) respectivement, pour XCyton ELISA et 75,0% (59,3-85,4) et 97,7% (93,3 -99,2) pour ELISA Panbio. Conclusion: 

Les deux tests ELISA disponibles dans le commerce ont des sensibilités et des spécificités raisonnablement excellentes pour le diagnostic de JEV.

Keywords: ELISA; Inde; India; Japanese encephalitis virus; diagnostic; diagnostics; diagnóstico; virus de la encefalitis japonesa; virus de l’encéphalite japonaise

Document Type: Research Article


Affiliations: 1:  Brain Infections Group, University of Liverpool, Liverpool, UK 2:  Vijayanagar Institute of Medical Sciences, Bellary, India 3:  Institute of Health and Community Medicine, Universiti Malaysia, Kota Samarahan, Malaysia 4:  National Institute of Mental Health and Neurological Sciences, Bangalore, India

Publication date: July 1, 2010


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