Free Content Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

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Abstract:

Summary Objective  To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods  Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results  The OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90–98.8%) and a specificity of 88.8% (95% CI: 81–93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67–87%) and 100% (95% CI: 96.3–100%), respectively. Conclusion  Our findings indicate high sensitivity of the OligoC-TesT on blood while the NASBA-OC is a better marker for active disease.

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Objectif: 

Estimer la sensibilité et la spécificité des tests OligoC et NASBA-OC pour la détection moléculaire de Leishmania dans le sang de patients LV confirmés et contrôles sains de zones endémiques du Kenya. Méthodes: 

Des échantillons de sang de 84 patients LV confirmés et 98 contrôles sains de zones endémiques dans le district de Baringo au Kenya ont été soumis aux deux tests. Résultats: 

Le test Leishmania OligoC a révélé une sensibilité de 96,4% (intervalle de confiance à 95% [IC]: 90% -98,8%) et une spécificité de 88,8% (IC95%: 81% -93,6%) tandis que la sensibilité et la spécificité du test NASBA-OC étaient de 79,8% (IC95%: 67% -87%) et 100% (IC95%: 96,3% -100%), respectivement. Conclusion: 

Nos résultats indiquent une sensibilitéélevée du test Leishmania OligoC sur le sang tandis que le test NASBA-OC est un meilleur marqueur de la maladie active.

Keywords: NASBA; PCR; diagnosis; diagnostic; diagnóstico; especificidad; leishmaniasis visceral; leishmaniose viscérale; nucleic acid sequence-based amplification; polymerase chain reaction; sensibilidad; sensibilité; sensitivity; specificity; spécificité; visceral leishmaniasis

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1365-3156.2010.02548.x

Affiliations: 1:  Centre for Clinical Research, Kenya Medical Research Institute, Nairobi, Kenya 2:  Royal Tropical Institute, KIT Biomedical Research, Amsterdam, The Netherlands 3:  Faculty of Medicine, University of Khartoum, Khartoum, Sudan 4:  Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda

Publication date: July 1, 2010

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