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Free Content Assessment of a 27-kDa antigen in Enzyme-Linked Immunosorbent Assay for the diagnosis of fasciolosis in Vietnamese patients

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Abstract:

Summary Fasciolosis has emerged as an important zoonotic disease in many parts of the world. In recent years, an increasing number of human cases were reported in Vietnam. In this study, the 27-kDa component protein from the excretory/secretory production of adult , purified by high performance liquid chromatography, was assessed in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against spp. for diagnosis of human fasciolosis. The ELISA showed a high sensitivity (100%) and specificity (97.67%) when tested on patients with fasciolosis, other parasitic infections, cholangiocarcinoma and on healthy controls. The assay was applied for diagnosis on 143 patients in the Viet Duc-Hanoi hospital who presented with clinical signs of liver disease and lesions in their livers as shown by imaging techniques. Antibodies were found in 37 (25.9%) of these patients, of whom only 3 shed eggs in their stools (2.1%). The excellent response to triclabendazole treatment of 37 sero-positive patients confirmed the diagnosis of fasciolosis. This study demonstrated the diagnostic potential for human fasciolosis of the 27-kDa antigen ELISA. Fasciolosis should be considered in the differential diagnosis of hepatic disease in Vietnam.

French
La fasciolose a émergé comme une zoonose importante dans de nombreuses régions du monde. Ces dernières années, un nombre croissant de cas humains ont été signalés au Vietnam. Dans cette étude, la composante protéine de 27 kDa du système de production excrétion/sécrétion des adultes de Fasciola gigantica, purifiée par chromatographie liquide haute performance, a étéévaluée dans un test ELISA afin de détecter les anticorps contre Fasciola spp. dans le diagnostic de la fasciolose humaine. Le test ELISA a montré une sensibilité (100%) et spécificité (97.67%) élevées lorsqu’il est testé sur des patients présentant la fasciolose, d’autres infections parasitaires, le cholangiocarcinome et sur des contrôles sains. Le test a été appliqué pour le diagnostic de 143 patients à l’hôpital de Viet Duc-Hanoi qui présentait des signes cliniques de maladie du foie et des lésions dans le foie tel que le montraient les techniques d’imagerie. Des anticorps ont été retrouvés chez 37 (25,9%) de ces patients, dont seuls 3 libéraient des oeufs de Fasciola dans leurs selles (2,1%). L’excellente réponse au traitement avec le triclabendazole de 37 patients séro-positifs a confirmé le diagnostic de la fasciolose. Cette étude a démontré le potentiel de diagnostic de la fasciolose humaine par ELISA sur l’antigène de 27 kDa. La fasciolose devrait être prise en compte dans le diagnostic différentiel de la maladie hépatique au Vietnam.

Keywords: ELISA; Enzyme-linked immunosorbent assay; Fasciolose; Fasciolosis; Vietnam; diagnosis; diagnostic; diagnóstico; enfermedad hepática; hepatic disease; inmunoensayo enzimático; maladie hépatique

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1365-3156.2010.02468.x

Affiliations: 1:  National Institute of Veterinary Research, Hanoi, Vietnam 2:  Department of Immunology, Institute of Biotechnology, Hanoi, Vietnam 3:  Department of Parasitology, Hanoi Medical University, Vietnam 4:  Viet Duc Hospital, Hanoi Medical University, Vietnam 5:  Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

Publication date: April 1, 2010

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