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Free Content Rapid colorimetric field test to determine levels of deltamethrin on PermaNet® surfaces: association with mosquito bioactivity

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Summary Objective  To evaluate a simple and inexpensive colorimetric test to measure the amount of cyanopyrethroid insecticide residue from filter paper exposed to mosquito net surfaces. Method  The net sampling protocol and colorimetric test (NetTest) were evaluated for deltamethrin-impregnated PermaNet 2.0 by comparison with high-performance liquid chromatographic assays and mosquito mortality (WHO Cone Test). Conclusion  The observed correlation between the amount of deltamethrin adsorbed onto the filter paper and the entire amount of deltamethrin per unit area of net material was good: 0.967, five assays. The relationship between the surface levels of deltamethrin determined by the colorimetric test and the ‘gold standard’ mosquito bioassay reveals a relatively accurate field test with a sensitivity of 91.4% and specificity if 85.4% (76 samplings from 19 nets).


Evaluer un test colorimétrique simple et peu coûteux pour mesurer la quantité de résidus d’insecticide cyanopyréthroide sur du papier filtre exposéà la surface de moustiquaires. Méthode: 

Le protocole d’échantillonnage sur les moustiquaires et du test colorimétrique (NetTest) a étéévalué pour le deltaméthrine imprégné PermaNet ® 2.0 en comparaison avec des tests de chromatographie haute performance en phase liquide et la mortalité des moustiques (Test OMS Cône). La corrélation observée entre la quantité de deltaméthrine adsorbée sur le papier filtre et la quantité totale de deltaméthrine par unité de surface de moustiquaire était bonne: 0.967  sur 5 essais. Conclusion: 

La relation entre les taux de deltaméthrine de surface déterminés par le test colorimétrique et le “gold standard” i.e la bioanalyse des moustiques révèle un test sur le terrain relativement précis avec une sensibilité de 91,4% et une spécificité de 85,4% (76 échantillons de 19 filets).
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Keywords: Malaria; bednet; cianopiretroide; cyanopyrethroid; cyanopyréthroide; deltamethrin; deltametrina; deltaméthrine; field test; malaria; moustiquaires; prueba de campo; redes mosquiteras; test sur le terrain

Document Type: Research Article

Affiliations: 1:  Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA USA 2:  Kenya Medical Research Institute, Kisumu, Kenya 3:  Center for Entomological Research, Cotonou, Benin

Publication date: 2009-04-01

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