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Free Content Sero-epidemiological study of kala-azar in a village of Varanasi district, India

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Abstract:

Summary Objective 

To evaluate five kala-azar serological tests for field use. Method 

Serological survey in Pandit Ka Purva village in Varanasi district, India, using Sia water test, aldehyde test, direct agglutination test (DAT), micro-enzyme-linked immunosorbent assay (ELISA) and dot-ELISA. Results 

The total population of the village was 518, 67 of whom showed typical clinical and parasitological features of kala-azar, including seven who died. The age distribution of kala-azar cases showed significant differences, being highest among the 45–54-year age group. The disease was more prevalent among males. Serum samples were collected from 498 persons (96% of total population) including 67 kala-azar cases and 40 disease controls (malaria, TB, leprosy, typhoid). Ten 10 serum samples from healthy controls living in endemic area were also collected. The test sensitivities were: Sia water test, 85.0%; aldehyde test, 62.7%; DAT, 94.0%; micro-ELISA, 91.0% and dot-ELISA, 97.0%. The test specificities were: Sia water test 92.5%, aldehyde test, 93.2%, DAT, 96.7; micro-ELISA, 97.6% and dot-ELISA, 98.4%. Conclusion 

The dot-ELISA is highly sensitive and specific, cheap, and easy to interpret with the naked eye, making it a powerful screening test for the surveillance and diagnosis of Indian kala-azar at field level.

Keywords: direct agglutination test; dot-ELISA; kala-azar; micro-ELISA; serodiagnosis

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1365-3156.2005.01538.x

Affiliations: 1: Department of Biochemistry, Banaras Hindu University, Varanasi, India 2: Kala-azar Medical Research Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India 3: Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

Publication date: 2006-01-01

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