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Free Content Falciparum malaria in the north of Laos: the occurrence and implications of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene haplotype SVMNT

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Abstract:

Summary Objective 

The Pfcrt-gene encodes a transmembrane protein located in the Plasmodium falciparum digestive vacuole. Chloroquine resistant (CQR) strains of African and Southeast Asian origin carry the Pfcrt-haplotype (c72–76) CVIET, whereas most South American and Papua New Guinean CQR stains carry the SVMNT haplotype. Method 

Eighty-eight samples from an area with reported in vivo Chloroquine and in vitro Amodiaquine-resistance were screened for the K76T mutation and their Pfcrt-haplotype (c72–76) using a new SSOP-ELISA. Results 

Hundred percent of the analysed samples showed the K76T mutation which is highly associated with in vivo drug failure. This very high rate of a CQR-marker is alarming in an area were CQ is still used as first line drug. The distribution of the three main Pfcrt-haplotypes was as follows: 68% CVIET, 31% SVMNT, 0% CVMNT. Conclusions 

These data show, for the first time, the South American/PNG -haplotype (SVMNT) on mainland Southeast Asia. SVMNT-haplotype and others might be associated with a decreased efficacy of Amodiaquine and could therefore be potential markers for of amodiaquine resistance (AQR). If there is a correlation between AQR and the SVMNT-haplotype as suggested, 31% prevalence of a potential resistance marker is cause for concern.

Keywords: CVIET; CVMNK; Pfcrt; SVMNT; amodiaquine; laos; malaria; resistance

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1365-3156.2005.01514.x

Affiliations: 1: Institute of Tropical Medicine, Berlin, Germany 2: Centre for Medical Parasitology at Institute of Medical Microbiology and Immunology and Institute of Public Health, University of Copenhagen, Denmark 3: Centre of Malariology, Parasitology and Entomology; Vientiane, Laos

Publication date: December 1, 2005

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