Free Content Short Communication: Palestinian infantile visceral leishmaniasis caused by a genetic variant of Leishmania infantum belonging to a new zymodeme

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Abstract:

Summary

The parasites causing a Palestinian case of infantile visceral leishmaniasis (IVL) and those from four dogs from the Jenin District were identified serologically, biochemically and molecular biologically as Leishmania infantum, showing dogs act as a reservoir. The strain from the human case was distinct because of its unique 200-bp kDNA-polymerase chain reaction (PCR) component in its restriction fragment length polymorphism (RFLP) profile after digestion with the endonuclease RsaI, and by the electrophoretic mobility of its malate dehydrogenase (MDH140), designating it the reference strain of a new zymodeme of L. infantum, MON-281.

Keywords: Leishmania infantum; Palestinian Authority; isoenzyme analysis; polymerase chain reaction

Document Type: Short Communication

DOI: http://dx.doi.org/10.1111/j.1365-3156.2005.01419.x

Affiliations: 1: Department of Parasitology, Hebrew University–Hadassah Medical School, Jerusalem, Israel 2: Laboratoire d'Ecologie Médicale et Pathologie Parasitaire et Centre National de Référence des Leishmania, Université de Montpellier I, Montpellier, France 3: Al-Watany Hospital, Nablus, West Bank, Palestinian Authority 4: Al-Quds University, Faculty of Public Health, West Bank, Palestinian Authority

Publication date: June 1, 2005

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