Skip to main content

Free Content Short Communication: Detection of dengue virus antigens in desiccated mosquitoes: an improved tool for surveillance

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Summary

We developed a surveillance tool to monitor dengue virus (DENV) infection in vector mosquitoes using a dengue antigen-capture enzyme immunoassay (EIA) on desiccated specimens stored at room temperature (RT). We tested the effect of storage on the stability of DEN1, DEN2, DEN3 and DEN4 antigens. Although desiccated infected Aedes aegypti mosquitoes stored between 31 and 34 °C (RT) showed greater reactivity in the EIA than those stored at −80 °C, a significant difference between the two was observed only with DEN2. Storage between 31 and 34 °C for up to 4 weeks (the longest period tested) did not affect the reactivity in the EIA, indicating the stability of DENV antigens.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: Aedes aegypti; dengue virus infection; desiccated mosquitoes

Document Type: Short Communication

Publication date: 2005-02-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more