Skip to main content

Free Content Short communication: Misleading microscopy in amoebiasis

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library



High prevalences of intestinal amoebiasis are commonly reported by microscopy in Ethiopia. In order to confirm the actual occurrence of Entamoeba histolytica we collected 108 stool specimens from different hospitals & health centers from patients in whom haematophagous trophozoites were believed to be found. We detected only a single E. histolytica case while 77 (71.3%) were E. dispar and the remaining 30 samples were negative for both species by real-time PCR based on the small subunit ribosomal RNA gene sequence of E. histolytica and E. dispar. The tradition of microscopy in a routine diagnostic set-up appears unsatisfactory to reliably differentiate rbc-engulfing amoeba from non-invasive amoeba in wet smears.

Keywords: Entamoeba dispar; Entamoeba histolytica; haematophagous; real-time polymerase chain reaction

Document Type: Short Communication


Affiliations: 1:  Ethiopian Health and Nutrition Research Institute (EHNRI)/Ethio-Netherlands AIDS Research Project (ENARP), Addis Ababa, Ethiopia 2:  Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands 3:  Department of Biology, Addis Ababa University (AAU), Addis Ababa, Ethiopia

Publication date: May 1, 2004


Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more