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Free Content Application of synthetic peptides to the diagnosis of neurocysticercosis

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Abstract:

Summary

We tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.

Keywords: Taenia solium; diagnosis; neurocysticercosis; peptides

Document Type: Research Article

DOI: https://doi.org/10.1046/j.1360-2276.2003.01132.x

Affiliations: 1: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F. Mexico 2: Instituto de Salud Carlos III, Majadahonda, Madrid, Spain 3: Department of Tropical Animal Health, University of Edinburgh, Roslin, UK 4: Instituto Gulbenkian de Ciencia, Oeiras, Portugal 5: Instituto Nacional de Neurología y Neurocirugía, México, D.F. México 6: Laboratório de Parasitología, Universidad Federal de Uberlandia, Uberlandia, Brazil 7: Faculdade de Medicina Veterinaria e Zootecnia, Universidad Estadual Paulista, Botucatu, Brazil 8: Faculdade de Medicina, Universidad Estadual Paulista, Botucatu, Brazil

Publication date: 2003-12-01

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