Skip to main content

Dengue virus infection rate in field populations of female Aedes aegypti and Aedes albopictus in Singapore

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

We developed a single-step reverse transcription-polymerase chain reaction (RT-PCR) followed by a seminested PCR using an upstream consensus primer and four type-specific primers within the non-structural protein gene (NS3) of dengue viruses to type dengue viruses in field populations of female Aedes mosquitoes. This yielded diagnostic fragments of 169, 362, 265 and 426 base pairs for dengue virus types 1, 2, 3 and 4, respectively. From 1997 to 2000, 54 (6.9%) of 781 Aedes aegypti and 67 (2.9%) of 2256 Aedes albopictus screened were positive for dengue viruses, with a declining trend. The most common dengue virus type detected in the Aedes mosquitoes was dengue-1. Details on the change of one serotype to another in the mosquito population over three consecutive years are discussed.

Keywords: Aedes aegypti; Aedes albopictus; dengue serotypes; dengue virus; polymerase chain reaction

Document Type: Original Article

DOI: https://doi.org/10.1046/j.1365-3156.2002.00873.x

Affiliations: Vector Control & Research Department, Ministry of the Environment, Singapore

Publication date: 2002-04-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more