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Short communication: Erythrocyte binding antigen (EBA-175) of Plasmodium falciparum: improved genotype determination by nested polymerase chain reaction

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We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large number of Plasmodium falciparum field isolates.
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Keywords: Plasmodium falciparum; erythrocyte binding antigen (EBA)-175 gene; genotype determination; nested polymerase chain reaction (PCR)

Document Type: Short Communication

Publication date: 2001-10-01

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