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Latex based, rapid and easy assay for human leptospirosis in a single test format

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Abstract:

Leptospirosis is an often severe disease which requires prompt treatment. Laboratory testing is required to reach a valid diagnosis. An agglutination assay for the detection of Leptospira-specific antibodies consisting of individually wrapped agglutination cards containing a stable, dried detection reagent is evaluated. The assay is simply performed by suspending the dried reagent with a drop of serum. The result is obtained within 30 s. The sensitivity of the assay varied with the stage of the disease and was 72.3% for samples collected during the first 10 days of the illness and 88.2% for samples collected at a later stage. The specificity was 93.9% and 89.8%, respectively. These characteristics make the test ideal for use in areas where the disease is common and where laboratory support is not routinely available.

Keywords: ELISA; latex agglutination assay; leptospirosis; microscopic agglutination assay; serodiagnosis

Document Type: Research Article

Affiliations: 1: Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands 2: Department of Dermatology, Academic Hospital, Paramaribo, Surinam 3: Malankara Orthodox Syrian Church Medical Mission Hospital, Kolenchery, India 4: Department of Medicine, Dr Kariadi Hospital/Diponegoro University, Semarang, Indonesia 5: Department of Medicine, Victoria Hospital, the Seychelles 6: Epidemiology Branch, Department of Health, Honolulu, Hawaii, USA 7: Department of Internal Medicine, Academic Hospital, Paramaribo, Surinam

Publication date: 2001-02-01

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