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Free Content Inhibition of glutathione synthesis as a chemotherapeutic strategy for leishmaniasis

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Abstract:

Summary

This study focuses on the use of buthionine sulphoximine (BSO), a γ-glutamylcysteine synthetase inhibitor, on Leishmania donovani growth. The effect of BSO on amastigote multiplication within macrophages showed that 5┬ámmBSO decreased infectivity by about 50% and the mean number of amastigotes per 100 infected macrophages by 21%. The mechanism may be that BSO resulted in enhanced nitric oxide (NO) levels within macrophages, probably due to inhibition of GSH content since GSH (10┬ámm) given after BSO treatment led to a decrease in NO compared to macrophages treated with BSO alone which were preexposed to the Leishmania surface molecule lipophosphoglycan.

Keywords: Leishmania donovani; glutathione; l-buthionine sulphoximine (BSO); nitric oxide

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1365-3156.2000.00565.x

Affiliations: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

Publication date: June 1, 2000

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