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Assessment of IgG antibodies against yellow fever virus after vaccination with 17D by different assays: neutralization test, haemagglutination inhibition test, immunofluorescence assay and ELISA

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We analysed serum samples of 209 subjects immunized with yellow fever vaccine 17D by different assays: neutralization test, immunofluorescence assay, haemagglutination inhibition test and ELISA, for presence of 17D-specific antibodies. Serum samples were taken from a few weeks up to 35 years after vaccination. The neutralization test had the highest sensitivity. There was no correlation of results between the serological assays. Considering NT titres > 1:10 as indicating protection, we found that about 75% of subjects remained immune even 10 years after vaccination, with a median NT titre of 1:40 in reactive sera.

Keywords: 17D yellow fever vaccination; ELISA; YF neutralization test; antibody persistence; haemagglutination inhibition test; immunofluorescence assay

Document Type: Research Article


Affiliations: 1: Robert Koch-Institut, Berlin, Germany 2: Medizinische Fakultät, Klinik und Poliklinik für Innere Medizin, Universität Rostock, Germany 3: Bernhard-Nocht-Institut, Hamburg, Germany

Publication date: December 1, 1999


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