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Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems

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Abstract:

Summary

The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both β-galactosidase and hepatitis B core antigen fusions or as a full-length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG-1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine.

Keywords: Theileria annulata; protection; sporozoite antigen (SPAG-1); vaccine

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1365-3156.1999.00453.x

Affiliations: 1: Department of Biology, University of York, UK 2: Centre for Tropical Veterinary Medicine, University of Edinburgh, UK 3: Roslin Institute, UK 4: International Livestock Research Institute, Kenya 5: Department of Parasitology and Tropical Veterinary Medicine, University of Utrecht, The Netherlands

Publication date: September 1, 1999

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