Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

Summary

The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both β-galactosidase and hepatitis B core antigen fusions or as a full-length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG-1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine.

Keywords: Theileria annulata; protection; sporozoite antigen (SPAG-1); vaccine

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1365-3156.1999.00453.x

Affiliations: 1: Department of Biology, University of York, UK 2: Centre for Tropical Veterinary Medicine, University of Edinburgh, UK 3: Roslin Institute, UK 4: International Livestock Research Institute, Kenya 5: Department of Parasitology and Tropical Veterinary Medicine, University of Utrecht, The Netherlands

Publication date: September 1, 1999

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more