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Population structure of recrudescent Plasmodium falciparum isolates from Western Uganda

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Abstract:

Summary

It has been proposed that polymorphisms of the Merozoite Surface Protein 1 and 2 (MSP1 and MSP2) and the Glutamate Rich Protein (GLURP) genes can be considered as genetic markers for the genotyping of field populations of Plasmodium falciparum. During a field study on in vivo drug resistance against chloroquine, sulphadoxine/pyrimethamine (S/P) and cotrimoxazole in West Uganda, sensitive and resistant isolates were collected from patients by fingerprick for genotyping. 59 (72.8%) of the 81 P. falciparum samples isolated at day 0 showed multiclonal infection with 2–7 clones. Among the isolates we investigated, presence of the allelic family MAD20 of MSP1 at day 0 was significantly (= 0.0041) associated with decreased resistance to antimalarials. Use of this method in a field study on in vivo drug resistance demonstrates another potential application of genotyping as a tool for epidemiological investigations.

Keywords: P. falciparum; drug resistance; epidemiology; genotyping; malaria; treatment

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1365-3156.1999.00428.x

Affiliations: 1: Department of Infectious Diseases and Tropical Medicine, University of Munich, Germany 2: Basic Health Services, GTZ, Fort Portal, Uganda

Publication date: July 1, 1999

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