Tandemly repeated genomic sequence demonstrates inter- and intra-strain genetic variation in Schistosoma japonicum*

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Abstract:

Genetic variability within and among four geographical strains of Schistosoma japonicum was examined using a novel repetitive element. The element, termed Sjrh1.0, was isolated from genomic DNA of a Philippine strain of S. japonicum using a combination of restriction fragment PCR and band-stab PCR. Sjrh1.0 is a tandemly repeated element, the sequence of which appears to be species-specific, in that it hybridized to DNA from S. japonicum but not to DNA from S. mansoni. Its sequence does not match previously deposited sequences in GenBank. When employed as a probe in Southern hybridization analysis, radiolabelled Sjrh1.0 revealed sex-specific and strain-specific differences in genomic DNA of individual worms. We also found individual genetic variation within geographical isolates of the Asian schistosome.

Keywords: Repetitive DNA; Schistosoma japonicum; strain vari

Document Type: Research Article

Affiliations: 1: Molecular Parasitology Unit, Queensland Institute of Medical Research, Brisbane, Queensland, Australia 2: Biomedical Research Institute, Rockville, Maryland, USA 3: Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA

Publication date: May 1, 1998

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